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Verlag GmbH
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GenScript corporation
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Image Search Results
Journal: Journal of Clinical Medicine
Article Title: Clinical Utility of Ghrelin-O-Acyltransferase (GOAT) Enzyme as a Diagnostic Tool and Potential Therapeutic Target in Prostate Cancer
doi: 10.3390/jcm8122056
Figure Lengend Snippet: Effects of GOAT in vitro and in vivo. Cell proliferation rate (determined by Alamar-Blue assay) at 24, 48 and/or 72 h in response to GOAT overexpression ( a ), silencing ( b ) and pharmacological blockade with the specific GOAT inhibitor GO-CoA-Tat ( c ). Results are referred as a percentage of the control condition (mock, scramble and vehicle-treated cells, respectively). ( d ) Comparison between the growth of xenograft tumors derived from mock-transfected cells (black line) and GOAT-overexpressing cells ( red line ) over time. ( e ) Number of mitosis per mm 2 in the xenograft tumors derived from mock-transfected DU145 cells and GOAT-overexpressing DU145 cells. ( f ) Percentage of positive KI67 cells in mock and GOAT xenograft tumors. ( g ) Representative image of tumor size of mock and GOAT xenograft tumors. ( h ) Representative image of xenograft tumors derived from mock-transfected DU145 cells and GOAT-overexpressing DU145 cells with hematoxylin-eosin staining. ( i ) Representative images of KI67 staining of xenograft tumors derived from mock-transfected DU145 cells and GOAT-overexpressing DU145 cells. The asterisks (*, p < 0.05; **, p < 0.01) indicate values that significantly differ between groups.
Article Snippet: A
Techniques: In Vitro, In Vivo, Alamar Blue Assay, Over Expression, Control, Comparison, Derivative Assay, Transfection, Staining
Journal: Translational Cancer Research
Article Title: ZBTB7B modulates the androgen receptor as an upstream regulator via colocalization and direct binding in LNCaP prostate cancer cells
doi: 10.21037/tcr-2025-1365
Figure Lengend Snippet: ZBTB7B depletion reduced viability, the number of colonies and increased G1 arrest in prostate cancer cells. (A,B) ZBTB7B expression levels at protein (A) and mRNA (B) levels in DU145 and LNCaP cells transfected with negative control siRNA and/or ZBTB7B siRNA plasmids. (C) Effects of ZBTB7B depletion on the viability of DU145 and LNCaP cells as determined by MTT assay. (D) Effects of ZBTB7B depletion on the number of colonies of DU145 and LNCaP cells. (E) Effects of ZBTB7B depletion on G1 arrest in DU145 and LNCaP cells as determined by FACS analysis. Graphs represent the percentage G1 arrest population. ***, P<0.001. FACS, fluorescence-activated cell sorting; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Article Snippet: LNCaP,
Techniques: Expressing, Transfection, Negative Control, MTT Assay, Fluorescence, FACS